mouse recombinant interleukin 17  (MedChemExpress)

Journal: Cell Host & Microbe

Article Title: Epithelial-Cell-Derived Phospholipase A 2 Group 1B Is an Endogenous Anthelmintic

doi: 10.1016/j.chom.2017.09.006

Figure Lengend Snippet:

Article Snippet: Recombinant mouse PLA2g1B protein , Elabscience , Cat# PKSM040953.

Techniques: In Vivo, Recombinant, ATP Assay, Software, Indirect Immunoperoxidase Assay

Journal: Cell Host & Microbe

Article Title: Epithelial-Cell-Derived Phospholipase A 2 Group 1B Is an Endogenous Anthelmintic

doi: 10.1016/j.chom.2017.09.006

Figure Lengend Snippet: Pla2g1b and Lipid Metabolism Correlate with Resistance to Intestinal Helminth Infection (A) C57BL/6 mice were orally infected with 200 L3 H. polygyrus ( H.p. ) larvae on day 0. A cohort of mice were sacrificed 7 days after 1° H.p. infection ( H.p. 1°). Remaining mice were drug treated (Rx) on days 14 and 15. Mice were then reinfected with H.p. on day 35 or day 56 and harvested 7 days after infection ( H.p. 2°). (B) H&E staining of the small intestine from H.p. 1° and H.p. 2° (D42). (C) Ratio-of-ratios analysis of differentially expressed genes in H.p. 1° and H.p. 2° (D42) identified distinct gene clusters (C1-3). (D) Top 10 pathways predicted to be activated more highly in and H.p. 2° than H.p. 1° (both relative to naive, 2-fold filter, p < 0.05). (E) Lipid metabolism pathway predicted activation score (relative to naive, 2-fold filter, p < 0.05). (F) Pla2g1b expression in small intestine from RNA sequencing data, confirmed by qPCR. Data are represented as mean ± SEM; n = 8, ∗ = p < 0.05, ∗∗ = p < 0.01 determined using a one-way ANOVA with Dunnett’s multiple comparison analysis. See also Figure S1 and .

Article Snippet: Recombinant mouse PLA2g1B protein , Elabscience , Cat# PKSM040953.

Techniques: Infection, Staining, Activation Assay, Expressing, RNA Sequencing, Comparison

Journal: Cell Host & Microbe

Article Title: Epithelial-Cell-Derived Phospholipase A 2 Group 1B Is an Endogenous Anthelmintic

doi: 10.1016/j.chom.2017.09.006

Figure Lengend Snippet: Type 2 Immunity Is Insufficient for Resistance to Intestinal Helminth Infection in the Absence of Pla2g1b (A) Luminal H.p. worms in the small intestine 14 days after 2° infection. (B) Fecal egg counts 14 days after 2° H.p. infection. (C) Frequency of Il4 gfp+ CD44 hi CD4 + cells in the mesenteric lymph node (mLN) and the spleen of mice 7 days after 2° infection. (D) Frequency of cytokine + CD4 + CD44 hi cells in the mLN of mice 7 days after 2° infection. (E) ex vivo H.p. antigen-specific cytokine production from the mLN of mice 7 days after 2° infection. (F) Gene expression in the small intestine of mice 7 days after 2° H.p. infection. (G) H.p. antigen-specific IgG1 in the serum from mice 7 days after 2° H.p. infection. (H) Mucus staining (Alcian blue-periodic acid-Schiff) of the small intestine from mice 7 days after 2° H.p. infection. (I) RNA-sequencing-generated transcriptional landscape of the small intestine of WT and Pla2g1b − /− mice 7 days after 2° H.p. infection. (J) Top 10 pathways predicted to be activated 7 days after 2° H.p. infection (relative to strain-naive, 2-fold filter, p < 0.05). (K) Lipid metabolism pathway predicted activation score 7 days after 2° H.p. infection (relative to strain-naive, 2-fold filter, p < 0.05). Data are represented as mean ± SEM, n = 5–6, representative of at least three independent experiments; ns = not significant, ∗ = p < 0.05, ∗∗∗∗ = p < 0.0001 determined using a two-way ANOVA with Sidak’s multiple comparison analysis or an unpaired two-tailed t test. See also .

Article Snippet: Recombinant mouse PLA2g1B protein , Elabscience , Cat# PKSM040953.

Techniques: Infection, Ex Vivo, Gene Expression, Staining, RNA Sequencing, Generated, Activation Assay, Comparison, Two Tailed Test

Journal: Cell Host & Microbe

Article Title: Epithelial-Cell-Derived Phospholipase A 2 Group 1B Is an Endogenous Anthelmintic

doi: 10.1016/j.chom.2017.09.006

Figure Lengend Snippet: Intestinal Pla2g1b Is Regulated by the Microbiota and Rag- and Common Gamma Chain-Dependent Cells in Resistant Mice (A) Kinetics of Pla2g1b expression in the small intestine during H.p. 2° infection model. (B) Kinetics of Pla2g1b expression in the small intestine during H.p. 1° infection, n = 6. (C) Pla2g1b expression at day 28 (14 days post-Rx) in the small intestine, n = 5. (D) Pla2g1b expression at day 28 (14 days post-Rx, 1°-Rx) in the small intestine following anti-IL-4 (αIL-4) or Isotype (Iso) treatment, n = 9–10 (data pooled from two independent experiments). (E) Pla2g1b expression at day 28 (14 days post-Rx, 1° Rx) in the small intestine, n = 7–10 (data pooled from two independent experiments). (F) Pla2g1b expression at day 28 (14 days post-Rx, 1° Rx) in the small intestine following antibiotic treatment, n = 9–10 (data pooled from two independent experiments). Data are represented as mean ± SEM, n = 3. All data are representative of at least two independent experiments; ∗ = p < 0.05, ∗∗ = p < 0.01, determined using a one-way ANOVA with Dunnett’s multiple comparison analysis, unpaired t test, or a Mann-Whitney test.

Article Snippet: Recombinant mouse PLA2g1B protein , Elabscience , Cat# PKSM040953.

Techniques: Expressing, Infection, Comparison, MANN-WHITNEY

Journal: Cell Host & Microbe

Article Title: Epithelial-Cell-Derived Phospholipase A 2 Group 1B Is an Endogenous Anthelmintic

doi: 10.1016/j.chom.2017.09.006

Figure Lengend Snippet: Epithelial-Cell-Derived Pla2g1b Is Negatively Regulated by IL-4Rα-Signaling (A) Pla2g1b detection by RNAScope ISH in the small intestine 7 days after 2° H.p. infection. (B) Pla2g1b expression in fluorescence-activated, cell sorted (FACS) CD45 – EpCam + and CD45 + EpCam − cells from the intestinal epithelium from naive and resistant (day 28) mice, n = 6. (C) Pla2g1b, Retnlb , and Gob5 expression in intestinal organoid cultures following stimulation with rIL-4 and rIL-13. Data are represented as mean ± SEM, n = 3. All data are representative of at least two independent experiments. ∗ = p < 0.05, ∗∗ = p < 0.01, ∗∗∗∗ = p < 0.0001 determined using an unpaired t test or a Mann-Whitney test. See also Figure S5 .

Article Snippet: Recombinant mouse PLA2g1B protein , Elabscience , Cat# PKSM040953.

Techniques: Derivative Assay, RNAscope, Infection, Expressing, Fluorescence, MANN-WHITNEY

Journal: Cell Host & Microbe

Article Title: Epithelial-Cell-Derived Phospholipase A 2 Group 1B Is an Endogenous Anthelmintic

doi: 10.1016/j.chom.2017.09.006

Figure Lengend Snippet: PLA 2 g1B Has Direct Anthelmintic Properties (A) ATP concentration of H.p. L3 larvae after 24 hr treatment with PLA 2 g1B, n = 3. (B) Number of H.p. larvae imbedded in the small intestinal wall 5 days after infection following 24 hr treatment with PLA 2 g1B, n = 5. (C) Luminal H.p. worms in the small intestine 14 days after 1° infection following 24 hr treatment with PLA 2 g1B, n = 10 (data pooled from two independent experiments). (D) Pla2g1b − /− or WT mice were orally infected with 200 L3 H.p. larvae on day 0 and were drug treated (Rx) on days 14 and 15. Mice were then reinfected with PLA 2 g1B-treated L3 H.p. larvae on day 35 and harvested 14 days after infection (2°). Another cohort of Pla2g1b − /− or WT mice were orally infected with 200 L3 H.p. larvae on day 35 and harvested 14 days after infection (1°). (E) Luminal H.p. worms in the small intestine 14 days after 1° or 2° infection following 24 hr treatment with PLA 2 g1B. (F) Luminal H.p. worms in the small intestine 14 days after 1° infection following 24 hr treatment with PLA 2 g1B. (G) Luminal H.p. worms in the small intestine 14 days after 2° infection following 24 hr treatment with PLA 2 g1B. Data are represented as mean ± SEM, n = 4–5. All data re representative of at least two independent experiments. ∗ = p < 0.05, ∗∗∗ = p < 0.001, ∗∗∗∗ = p < 0.0001 determined using a two-way ANOVA with Sidak’s multiple comparison analysis, one-way ANOVA with Dunnett’s multiple comparison analysis, or an unpaired two-tailed t test. See also Figure S6 .

Article Snippet: Recombinant mouse PLA2g1B protein , Elabscience , Cat# PKSM040953.

Techniques: Concentration Assay, Infection, Comparison, Two Tailed Test

Journal: Cell Host & Microbe

Article Title: Epithelial-Cell-Derived Phospholipase A 2 Group 1B Is an Endogenous Anthelmintic

doi: 10.1016/j.chom.2017.09.006

Figure Lengend Snippet: Pla2g1b-Treatment Related Changes in Lipid Abundance Relative abundances of phosphatidylethanolamine (PE) and other lipids extracted from PLA 2 g1B-treated (10 ng/μL) and control-treated (0 ng/μL) larvae. (A) Identified PEs. Features were regarded as “identified” by comparison of their precursor ion and MS/MS fragments with the LipidBlast library, as outlined in Figure S1 . The arrangement of fatty acid moieties on the glycerol backbone (i.e., whether in the sn- 1 or sn- 2 position) and the position of double bonds could not be inferred. PE 38:3 and PE 38:4 were detected as a mixture of different fatty acid moieties. (B) Putatively identified (annotated) PEs. The features could be “annotated” as PEs by comparison of peak retention time and inter-cluster mass shifts of 28 Da (CH 2 CH 2 ) and intra-cluster mass shifts of 2 Da (indicative of difference in double bond number [fatty acid saturation]) with other, identified PEs. MS/MS could not be performed due to low abundance. Data are shown as normalized intensities expressed in arbitrary units. Data are represented as mean ± SEM, n = 3. ∗ = p < 0.05. TIC: Total ion current. See also Figure S7 .

Article Snippet: Recombinant mouse PLA2g1B protein , Elabscience , Cat# PKSM040953.

Techniques: Control, Comparison, Tandem Mass Spectroscopy

Journal: Cell Host & Microbe

Article Title: Epithelial-Cell-Derived Phospholipase A 2 Group 1B Is an Endogenous Anthelmintic

doi: 10.1016/j.chom.2017.09.006

Figure Lengend Snippet:

Article Snippet: Recombinant mouse PLA2g1B protein , Elabscience , Cat# PKSM040953.

Techniques: In Vivo, Recombinant, ATP Assay, Software, Indirect Immunoperoxidase Assay